Handbook

Sequence Composition

The frequency of DNA bases, observed with emphasis on GC-content, can be a useful metric for evolutionary biologists. For example, GC-content within the barcoding region of CO1 has been correlated with GC-content of the entire mitochondrial genome for many species.

Parameters

Various distance models and alignment algorithms are available as parameters, as well as options to filter out sequences based on sequence length or sequence issues. The default parameters allow for GC percentages to be calculated on overall sequence composition as well as codon positions 1, 2 and 3 - but these may be unselected if desired.

Results

The results page provides statistics on the frequency of each base (G, C, A and T) in the selected records and can display histograms for GC content on all codon positions.

Sequence Composition results page